接第二篇
4.5. Rigidoporus piceicola (B.K. Cui & Y.C. Dai)F. Wu, jia J. Chen & Y.C. Dai, mycologia 109 (5):761 (2017) [MB#819208]
Basidiocarps annual, effused-reflexed, pileate,mostly imbricate, soft when fresh, becoming woody hard when dry (Figures 1(F,G) and 2(E)). Pileus broadly attached, up to 5 cm wide, 4 cm long, and 3.5 cm thick at the base. Pileus surface very pale brown (10YR, 8/3) to reddish yellow (7.5YR, 8/6) when fresh, very pale yellow (2.5Y, 9.5/2) to margin yellow (2.5Y, 7/8) when dry, azonate, margin sinuate. Pores angular to round, 4–5 per mm, dissepiments thin, entire. Pore surface white (2.5Y, 8/1) to yellow (2.5Y, 8/6). Context pale brown (2.5Y, 8/2),corky, azonate, up to 5mm thick. Tubes concolorous with pore surface, each layer up to 1.5mm long, tube layers distinct. Hyphal system monomitic, hyphae usually septate and without clamp connections, occasionally branched, thin-walled,with round apex, hyaline, 2.5–4.0 mm in diam. Basidia clavate, 4-spored, smooth, hyaline,11.5–133.5–4.0 mm (n=30). Basidiospores ellipsoid,smooth, thin-walled, hyaline, sometimes containing a guttule (2.5–)3.0–4.5×2.0–3.0(–3.5) μm[Q=(1.25–)1.3–1.5, Q=1.4 ± 0.15] (n=38).Cystidia arising from the trama, clavate, apically encrusted with coarse crystals, thick-walled, hyaline(19–)20.5 – 25.5(–28)4.0–5.0 μm (n=42).
4.5.1. Specimen examined
Korea. Gwangju Metropolitan City, Mudeungsan National Park, 35°07'52ʺ N, 126°59'62ʺ E, mixed hardwood forest, clustered or mesic in the lower part of a broad-leaved tree stump, 24 Sep 2020, S. L.Kwon, KUC20200924-53 (NIBRFG0000511373).
4.5.2. Remarks
Rigidoporus piceicola is characterized by thick-walled and apically encrusted cystidia with coarse crystals, and ellipsoid thin-walled basidiospores. The microscopic characteristics of Ri. piceicola KUC20200924-53 are similar to Ri. piceicola (B.K. Cui & Y.C. Dai)F. Wu, Jia J. Chen & Y.C. Dai (basionym: Oxyporus piceicola) [20,58]. However, the basidiospore size of KUC20200924-53 is smaller than previously reported [58]. Although Ri. piceicola is parasitic only on gymnosperm trees mostly on the genus Picea in the original description, it was found on a broad-leaved tree stump in this study. Based on phylogenetic analysis, Rigidoporus populinus (Fr.) Donk is the most closely related species. Ri. populinus has clavate basidia and apically encrusted cystidia, like Ri. piceicola. However, the former could be distinguished by subglobose and thick-walled basidiospores, and shorter cystidia (10–15×4.0–5.0 μm)[59]. In addition, Ri. subpopulinus is similar with Ri.piceicola by having imbricate basidiocarps and ellipsoid basidiospores, but the former has shorter basidia (7.6–12×5–6 μm) [60]. Because of the structural similarities in Rigidoporus, it is highly recommended that the DNA-based identification with combined regions, ITS, LSU, and rpb2.
4.6. Russula raoultii quél., comptes rendus de lassociation franc¸aise pour lavancement des sciences 14 (2): 449 (1886) [MB#208070]
Pileus largely depressed center, 20mm in diam, soft when fresh, becoming woody hard when dry; Pileus surface smooth, white (2.5Y, 9.5/1) to cream (2.5Y,9/2), pale yellow (2.5Y, 8/2) in the center; Margin entire, rounded-obtuse, incurved. Lamellae adnate, subclose to close, pale orange yellow (10YR, 9.5/2)to very pale brown (10YR, 8/4) (Figures 1(H) and 2(F)). Stipe cylindrical, soft-cottony, white (2.5Y,9.5/1), 25×10 mm. Hyphal system monomitic, hyphae occasionally septate and without clamp connections,mostly branched, thin-walled, becoming narrower toward the end with round apex, hyaline,1.8–4.3 μm in diam. Basidia clavate, 4-spored,smooth, hyaline, 30–36.5×8.0–8.5 μm (n=25).Basidiospores broadly ellipsoid, napiform, striate with variable ridges, thin-walled, hyaline, sometimes bearing a big guttle (5.0–)5.5–6.0(–7.0)×4.0–5.0(–5.5) μm [Q = (1.2–)1.25–1.27(–1.38), Q=1.26 ±0.1] (n=44). Cystidia mainly fusiform, sometimes capitate, clavate, hyphoid, smooth, thin-walled, hyaline,25–67×3.3–12 μm (n=39).
4.6.1. Specimen examined
Korea. Gwangju Metropolitan City, Mudeungsan National Park, mixed hardwood forest, 35°08'26ʺ N,126°58'00ʺ E, solitary on soil covered with woody debris, 16 Jun 2020, S. L. Kwon, KUC20200616-18(NIBRFG0000511375).
4.6.2. Remarks
Russula raoultii KUC20200616-18 was well-matched(99%) with Ru. raoultii Quel in the phylogenetic analysis using the LSU region. For an accurate analysis,additional regions must be identified.Microscopic characteristics of Ru. raoultii KUC20200616-18 matched the original description [61].However, the basidiospore of KUC20200616-18 is smaller than the size described in the original description (7.0–8.0×5.7–7.0 μm).
4.7. Scutellinia crinita (bull.) Lambotte,Mémoires de la société royale des sciences de liège, sér. 2 14 (7): 299 (1887) [MB#433534]
Apothecia sessile, scattered to gregarious, discoid,undulate; Disk round, 2.5–4mm in diam; Margin distinct, covered by blackish brown (7.5YR, 2.5/2) hairs which are stretched inward and outward(Figures 1(I) and 2(G)). Hymenial surface smooth,ceraceous, orange (7.5YR, 7/8) when fresh, pale yellow(5Y, 8/4) when dry. Marginal hair acicular,stiff, with pointed apex, 10–12 septate, thick-walled,brown (7.5YR, 4/4) to dark brown (7.5YR, 3/2),1000–2300×24–80 μm. Internal hair similar with marginal hair, but much shorter, < 300 μm. Asci cylindrical, with round apex, 8-spored, smooth,thin-walled, hyaline, 98–14012.5–14 mm (n=28).Ascospores ellipsoid to cylindrical, pustule-cristate,uniseriate, thin-walled, hyaline, containing two guttules,17–19.5(–21)×(9.0–)10–13(–13.5) μm [Q =(1.5–)1.6–1.7(–1.9), Q=1.65 ± 0.15] (n=40).
4.7.1. Specimen examined
Korea. Gwangju Metropolitan City, Mudeungsan National Park, 35°07'84ʺ N, 126°59'26ʺ E, mixed hardwood forest, occurring on rotten branches, 23 Sep 2020, S. L. Kwon, KUC20200923-07 (NIBRFG0000511370).
4.7.2. Remarks
Scutellinia crinita is similar to S. scutellata in terms of morphological characteristics, geographic distribution, and substrate [51]. Scutellinia crinita KUC20200923-07 has shorter asci and longer marginal hairs than S. scutellata [62]. Notably,Scutellinia species with globose to subglobose ascospores were found in soil. However, other Scutellinia species with ellipsoid ascospores have been found on decayed wood. This suggests that substrates may be crucial for understanding the diversity of the genus Scutellinia [50].
Acknowledgments
The authors thank Editage (www.editage.co.kr) for English language editing.
Disclosure statement
Y.M. Heo is employed by COSMAX BTI.
The rest of the authors declare that the research was conducted in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest.
Funding
This research was supported by the project on Acquisition and characterization of extremophile (II-2) of the National Institute of Biological Resources[NIBR202203112] under the Ministry of Environment,Republic of Korea. And this study was also supported by a Korea University Grant.
ORCID
Jae-Jin Kim http://orcid.org/0000-0001-8990-2139